Wednesday, March 28, 2007

Don't use eGFP.


PLoS ONE has a great paper on eGFP overexpression inhibition of polyubiquitination both through Lysines 63 & 48.
Overexpression of this super popular fluorescent reporter protein effects polyubiquitination and the subsequent digestion via the proteasome to a degree that it signifantly stabilizes p53 and increases p53 downstream signalling.
Crazy. I wonder how it inhibits ubiquitination.


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