Holy multiplexing batman!

CDKN2A which inactivates both RB and P53 is aparently the most common chromosomal deletion in cancer. It is therefore of interest to use this as a diagnostic marker. But how do you detect a rare mutated DNA in a very large pool of normal DNA? Well you multiplex your PCR! These guys who just published in PLOS ONE have a new multiplex method "Primer Approximation Multiplex PCR (PAMP), for enriching breakpoint sequences followed by genomic tiling array hybridization to locate the breakpoints" . When they say multiplex they don't joke... It's has no less than 7 primer pairs!