Tuesday, April 17, 2007

Holy multiplexing batman!


CDKN2A which inactivates both RB and P53 is aparently the most common chromosomal deletion in cancer. It is therefore of interest to use this as a diagnostic marker. But how do you detect a rare mutated DNA in a very large pool of normal DNA? Well you multiplex your PCR! These guys who just published in PLOS ONE have a new multiplex method "Primer Approximation Multiplex PCR (PAMP), for enriching breakpoint sequences followed by genomic tiling array hybridization to locate the breakpoints" . When they say multiplex they don't joke... It's has no less than 7 primer pairs!


0 comments: